Journal of Clinical Oncology, Vol 11, 1344-1352, Copyright © 1993 by American Society of Clinical Oncology
Detection of occult lymphoma in the peripheral blood and bone marrow of patients with untreated early-stage and advanced-stage follicular lymphoma
NL Berinstein, MD Reis, BY Ngan, CA Sawka, HH Jamal and B Kuzniar
Department of Medicine, Immunology, University of Toronto, Ontario, Canada.
PURPOSE: The object of this study was to compare the relative sensitivities
of morphologic, immunophenotypic, gene rearrangement, cytogenetic, and
polymerase chain reaction (PCR) analyses in the detection of lymphoma cells
in the bone marrow and peripheral blood of patients with follicular
lymphoma. PATIENTS AND METHODS: Bone marrow and peripheral-blood samples
from 28 newly diagnosed patients with follicular lymphoma referred from
several different medical centers were assessed. Routine morphologic
assessment was performed initially and the remainder of the sample was
aliquoted for DNA extraction to be used for gene rearrangement and PCR
analyses and for immunophenotypic and cytogenetic analyses where a
sufficient amount of sample remained. RESULTS: Morphologic assessment of
the bone marrow was positive for lymphoma cells in 11 of 28 patients. PCR
amplification of t(14;18) breakpoint DNA detected lymphoma cells in 17 of
24 patients assessed. Morphologic assessment detected lymphoma cells in
three bone marrow samples that were negative by PCR. PCR analysis was the
only method able to detect circulating lymphoma cells in peripheral blood
at diagnosis and was positive in 15 of 24 samples. The other methods of
assessment did not show lymphoma in any samples in which lymphoma was not
detected by morphologic or PCR analysis. Lymphoma cells were found in the
bone marrow and/or peripheral blood as frequently in early-stage patients
as in advanced-stage patients. CONCLUSION: PCR amplification of t(14;18)
breakpoint DNA together with morphologic assessment had the highest yield
of detecting lymphoma cells in the bone marrow and/or peripheral blood of
our population of newly diagnosed patients with follicular lymphoma. The
clinical significance and prognostic importance of lymphoma cells detected
by PCR in the bone marrow and/or peripheral blood of newly diagnosed
follicular lymphoma patients awaits long-term follow-up data of these and
additional patients.
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Facebook  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
R. J. Jones, C. D. Gocke, Y. L. Kasamon, C. B. Miller, B. Perkins, J. P. Barber, M. S. Vala, J. M. Gerber, L. L. Gellert, M. Siedner, et al.
Circulating clonotypic B cells in classic Hodgkin lymphoma
Blood,
June 4, 2009;
113(23):
5920 - 5926.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Jacobs, S. H. Swerdlow, J. Kant, K. A. Foon, R. Jankowitz, S. R. Land, N. DeMonaco, J. Joyce, J. L. Osborn, T. L. Evans, et al.
Phase II Trial of Short-Course CHOP-R Followed by 90Y-ibritumomab Tiuxetan and Extended Rituximab in Previously Untreated Follicular Lymphoma
Clin. Cancer Res.,
November 1, 2008;
14(21):
7088 - 7094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. K. Weinberg, W. Z. Ai, M. R. Mariappan, C. Shum, R. Levy, and D. A. Arber
''Minor'' BCL2 Breakpoints in Follicular Lymphoma: Frequency and Correlation with Grade and Disease Presentation in 236 Cases
J. Mol. Diagn.,
September 1, 2007;
9(4):
530 - 537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Biagi and J. F. Seymour
Insights into the molecular pathogenesis of follicular lymphoma arising from analysis of geographic variation
Blood,
May 29, 2002;
99(12):
4265 - 4275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Summers, L. K. Goff, A. G. Wilson, R. K. Gupta, T. A. Lister, and J. Fitzgibbon
Frequency of the Bcl-2/IgH Rearrangement in Normal Individuals: Implications for the Monitoring of Disease in Patients With Follicular Lymphoma
J. Clin. Oncol.,
January 15, 2001;
19(2):
420 - 424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lopez-Guillermo, F. Cabanillas, T. I. McDonnell, P. McLaughlin, T. Smith, W. Pugh, F. Hagemeister, M. A. Rodriguez, J. E. Romaguera, A. Younes, et al.
Correlation of Bcl-2 Rearrangement With Clinical Characteristics and Outcome in Indolent Follicular Lymphoma
Blood,
May 1, 1999;
93(9):
3081 - 3087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lopez-Guillermo, F. Cabanillas, P. McLaughlin, T. Smith, F. Hagemeister, M. A. Rodriguez, J. E. Romaguera, A. Younes, A. H. Sarris, H. A. Preti, et al.
The Clinical Significance of Molecular Response in Indolent Follicular Lymphomas
Blood,
April 15, 1998;
91(8):
2955 - 2960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Leonard, F. Hetu, L. Busque, M. Gyger, R. Belanger, C. Perreault, and D.-C. Roy
Lymphoma Cell Burden in Progenitor Cell Grafts Measured by Competitive Polymerase Chain Reaction: Less Than One Log Difference Between Bone Marrow and Peripheral Blood Sources
Blood,
January 1, 1998;
91(1):
331 - 339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
