Journal of Clinical Oncology, Vol 15, 583-588, Copyright © 1997 by American Society of Clinical Oncology
Detection of circulating tumor cells in patients with Ewing's sarcoma and peripheral primitive neuroectodermal tumor
DC West, HE Grier, MM Swallow, GD Demetri, L Granowetter and J Sklar
Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. dcwest@ucdavis.edu
PURPOSE: To determine the feasibility of detecting Ewing's sarcoma (ES) or
peripheral primitive neuroectodermal tumor (PNET) through a reverse-
transcriptase polymerase chain reaction (RT-PCR) of the t(11;22)(q24;q12)
fusion transcript in blood and bone marrow samples from patients with these
neoplasms. PATIENTS AND METHODS: Peripheral- blood (PB) and/or bone marrow
aspirate (BM) samples were obtained from 28 patients with ES or PNET at
initial presentation or at relapse. Patients were divided into two groups:
newly diagnosed patients with nonmetastatic disease and those with
metastatic/relapsed disease. RNA was extracted from fractionated BM and PB
samples, and RT-PCR was performed for the EWS/HumFLI1 fusion mRNA was
transcribed across the t(11;22) breakpoint. RESULTS: Among the 16 patients
with nonmetastatic disease, three of 16 were RT-PCR positive for
EWS/HumFLI1 RNA in BM and three of 10 were positive in PB. The total number
of nonmetastatic patients who were positive in either PB or BM was four of
16 (25%). Among patients with metastatic/relapsed disease, two of six were
positive in BM and five of 10 were positive in PB. The total fraction of
patients with metastatic/relapsed disease that was positive in either BM or
PB was six of 12 (50%). CONCLUSION: In this study, we show that it is
possible to amplify the EWS/HumFLI1 RNA by RT-PCR from the BM and PB of a
subset of patients with both nonmetastatic and metastatic ES or PNET, which
implies that occult tumor cells are present at these sites. The true
biologic and clinical meaning of this information is unknown. However, it
does suggest a possible application of RT-PCR for the monitoring of
residual disease in patients who are undergoing therapy for ES or PNET.
This approach may permit early identification of patients who may benefit
from alternative therapy or who may be spared possible overtreatment.
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Facebook  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
I. Y. Cheung, Y. Feng, K. Danis, N. Shukla, P. Meyers, M. Ladanyi, and N.-K. V. Cheung
Novel Markers of Subclinical Disease for Ewing Family Tumors from Gene Expression Profiling
Clin. Cancer Res.,
December 1, 2007;
13(23):
6978 - 6983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Chang and V. B. Shidham
Molecular Genetics of Pediatric Soft Tissue Tumors: Clinical Application
J. Mol. Diagn.,
August 1, 2003;
5(3):
143 - 154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S A Burchill
Ewing's sarcoma: diagnostic, prognostic, and therapeutic implications of molecular abnormalities
J. Clin. Pathol.,
February 1, 2003;
56(2):
96 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Schleiermacher, M. Peter, O. Oberlin, T. Philip, H. Rubie, F. Mechinaud, D. Sommelet-Olive, J. Landman-Parker, D. Bours, J. Michon, et al.
Increased Risk of Systemic Relapses Associated With Bone Marrow Micrometastasis and Circulating Tumor Cells in Localized Ewing Tumor
J. Clin. Oncol.,
January 1, 2003;
21(1):
85 - 91.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bertuzzi, L. Castagna, A. Nozza, V. Quagliuolo, L. Siracusano, M. Balzarotti, S. Compasso, M. Alloisio, H. Soto Parra, and A. Santoro
High-Dose Chemotherapy in Poor-Prognosis Adult Small Round-Cell Tumors: Clinical and Molecular Results From a Prospective Study
J. Clin. Oncol.,
April 15, 2002;
20(8):
2181 - 2188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Merino, F. Navid, B. L. Christensen, J. A. Toretsky, L. J. Helman, N.-K. V. Cheung, and C. L. Mackall
Immunomagnetic Purging of Ewing's Sarcoma From Blood and Bone Marrow: Quantitation by Real-Time Polymerase Chain Reaction
J. Clin. Oncol.,
August 15, 2001;
19(16):
3649 - 3659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Meyers, M. D. Krailo, M. Ladanyi, K.-W. Chan, S. L. Sailer, P. S. Dickman, D. L. Baker, J. H. Davis, R. B. Gerbing, A. Grovas, et al.
High-Dose Melphalan, Etoposide, Total-Body Irradiation, and Autologous Stem-Cell Reconstitution as Consolidation Therapy for High-Risk Ewing's Sarcoma Does Not Improve Prognosis
J. Clin. Oncol.,
June 1, 2001;
19(11):
2812 - 2820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. de Alava and J. Pardo
Ewing Tumor: Tumor Biology and Clinical Applications
International Journal of Surgical Pathology,
January 1, 2001;
9(1):
7 - 17.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W. L. Gerald
A Practical Approach to the Differential Diagnosis of Small Round Cell Tumors of Infancy Using Recent Scientific and Technical Advances
International Journal of Surgical Pathology,
April 1, 2000;
8(2):
87 - 97.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. de Alava and W. L. Gerald
Molecular Biology of the Ewing’s Sarcoma/Primitive Neuroectodermal Tumor Family
J. Clin. Oncol.,
January 5, 2000;
18(1):
204 - 204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Ghossein, S. Bhattacharya, and J. Rosai
Molecular Detection of Micrometastases and Circulating Tumor Cells in Solid Tumors
Clin. Cancer Res.,
August 1, 1999;
5(8):
1950 - 1960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
