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Proteasome Inhibition With Bortezomib (PS-341): A Phase I Study With Pharmacodynamic End Points Using a Day 1 and Day 4 Schedule in a 14-Day Cycle

From the New York University School of Medicine, New York, NY; Dana-Farber Cancer Institute; Massachusetts General Hospital, Boston, MA; Cancer Therapy Evaluation Program, National Cancer Institute, National Institutes of Health, Bethesda, MD; Millennium Pharmaceuticals Inc, Cambridge, MA

Address reprint requests to Franco M. Muggia, MD, New York University Medical Center, 550 First Ave, Suite 9R, New York, NY 10016; e-mail: muggif01{at}gcrc.med.nyu.edu

PURPOSE: We performed a phase I study of a day (D) 1 and D4 bortezomib administration once every 2 weeks to determine the recommended phase II dose and toxicity profile, and the extent of 20S proteasome inhibition obtained.

PATIENTS AND METHODS: Patients with solid tumors or lymphomas were treated with bortezomib at 0.25 to 1.9 mg/m² on D1 and D4, every 2 weeks. 20S proteasome levels in blood were assayed at baseline and at 1, 4, and 24 hours postdose in cycle 1.

RESULTS: On this D1 and D4 every 2 weeks' schedule, dose-limiting toxicity (DLT) was evident at the 1.75 and 1.9 mg/m² dose levels, most commonly in patients receiving individual total doses 3.0 mg. The main DLT was peripheral neuropathy evident at the higher doses and in patients previously exposed to neurotoxic agents. Other DLTs included diarrhea and fatigue; grade 3 thrombocytopenia was also noted. Reversible inhibition of 20S proteasome activity was dose dependent and best fit a total dose (mg) per fraction rather than mg/m²; 70% of baseline activity was inhibited by a dose of 3.0 to 3.5 mg given on D1 and on D4 every other week. Antitumor effects short of confirmed partial responses were observed in patients with melanoma, non–small-cell lung cancer, and renal cell carcinoma.

CONCLUSION: Bortezomib (PS-341) is a novel antineoplastic agent that is well tolerated at doses not exceeding 3.0 mg (equivalent to 1.75 mg/m²), repeated on D1 and D4 every other week. This dose correlates with 70% inhibition of 20S proteasome activity. DLTs include neuropathy, fatigue, and diarrhea.

Supported by research grants from the National Cancer Institute: U01 CA76642, M01 RR00096, and P30 CA16087 (New York University) and U01 62490 (Dana-Farber Cancer Institute/Massachusetts General Hospital).

Authors' disclosures of potential conflicts of interest are found at the end of this article.

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