Journal of Clinical Oncology, Vol 4, 278-283, Copyright © 1986 by American Society of Clinical Oncology
Diagnosis of B cell lymphoma by analysis of immunoglobulin gene rearrangements in biopsy specimens obtained by fine needle aspiration
E Hu, S Horning, S Flynn, S Brown, R Warnke and J Sklar
Histologic diagnosis of lymphoma is far more difficult in the disaggregated
cells obtained by percutaneous aspiration of lymph nodes than in tissue
sections prepared from excisional biopsy specimens. However, the
simplicity, economy, and safety of aspiration biopsy makes this an
attractive diagnostic option in certain situations. In the present study,
we demonstrate that lymph node aspirates provide material that is both
suitable and sufficient for accurately detecting clonal proliferations of B
cells by analysis of immunoglobulin gene rearrangements. The rearrangements
detected in aspirated tissue serve as clonal markers that can be directly
compared with the rearrangements found in histologically confirmed lymphoma
removed by open biopsy. The application of gene rearrangements to aspirated
material therefore offers a useful method of diagnosing lymphoma,
particularly for the purposes of more thorough staging at initial
presentation or the evaluation of tissues for possible relapse.
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